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Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.

A DNA oligonucleotide end-labeled with IRDye 700 infrared dye is a good substrate for protein binding. Using the Odyssey Infrared Imaging System, IRDye infrared dye labeled DNA detection is linear within a 50-fold dilution range from 9.1 fmol to 0.18 fmol. Infrared assays can be completed in less than two hours with no gel transfer or film exposure. The gel doesn’t even have to be removed from the glass plates for imaging. Plus, the gel can be placed back into the electrophoresis unit and run longer, if necessary.

IRDye 700 infrared dye-labeled EMSA oligonucleotides are supplied as 25 μl of 50 nM double-stranded DNA

Please note: Oligos with an * require up to two weeks for processing and delivery.

Part #	Description	Downloads	Order Online
829-07910	EMSA Buffer Kit for the Odyssey	description msds protocol	$ 195.00
829-07925	EMSA Oligo, AP-1 (IRDye 700)	msds pack insert	$ 50.00
829-07933	EMSA Oligo, ARE (Androgen Receptor) (IRDye 700)*	msds pack insert	$ 50.00
829-07923	EMSA Oligo, CREB (IRDye 700)*	msds pack insert	$ 50.00
829-07924	EMSA Oligo, NFkB (IRDye 700)	msds pack insert	$ 50.00
829-07926	EMSA Oligo, Sp-1 (IRDye 700)	msds pack insert	$ 50.00
829-07922	EMSA Oligo, STAT3 (IRDye 700)*	msds pack insert	$ 50.00

Part # Description Downloads Order Online 829-07910 EMSA Buffer Kit for the Odyssey description msds protocol $ 195.00
Tube	Reagent (Reagents for 100 EMSA Reactions)	Volume
1	10 X Binding Buffer (100mM Tris, 500mM KCl, 10mM DTT; pH 7.5)	500ul
2	25mM DTT/ 2.5% Tween-20	500ul
3	Poly (dI-dC) (1ug/ul) in 10mM Tris, 1 mM EDTA, pH 7.5	125ul
4	Sheared Salmon Sperm DNA (0.5 ug/ul) in 10mM Tris, 1 mM EDTA, pH 7.5	125ul
5	50% Glycerol	500ul
6	1% NP-40	500ul
7	1M KCl	500ul
8	100mM MgCl2	500ul
9	200mM EDTA, pH 8	500ul
10	10X  Orange Loading Dye	500ul
Storage: -20°C

Shipping information: U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST. Canada: Allow 3-5 days for shipments. Germany & UK: Contact LI-COR GmbH. International: Contact a LI-COR distributor in your area. All shipments subject to availability.