Infrared fluorescence detection has changed Western blotting (immunoblotting) by providing unparalleled quantification accuracy and simultaneous two-color multiplexed detection. Simultaneous two-color detection allows detection of both total protein as well as the amount of protein phosphorylation without stripping and reprobing.

LI-COR’s Western blotting kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey and Aerius infrared imaging systems. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes. Secondary antibodies in each kit are cross-absorbed for multiplex detection and provide high specificity.

Kits with PVDF Membranes

IRDye Western Kit I (part #926-31050)
• IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 μl, 1mg/ml)
• IRDye 680 Goat Anti-Rabbit Secondary Antibody (25 μl, 1mg/ml)
• 500 ml Odyssey Blocking Buffer
• 10 Millipore Immobilon™-FL PVDF Membranes (0.45 μm, 10 x 10 cm)

IRDye Western Kit II (part #926-31052)
• IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 μl, 1mg/ml)
• IRDye 680 Goat Anti-Mouse Secondary Antibody (25 μl, 1mg/ml)
• 500 ml Odyssey Blocking Buffer
• 10 Millipore Immobilon-FL PVDF Membranes (0.45 μm, 10 x 10 cm)

Kits with Nitrocellulose Membranes

IRDye Western Kit III (part #926-31058)
• IRDye 800CW Goat Anti-Mouse Secondary Antibody (25 μl, 1mg/ml)
• IRDye 680 Goat Anti-Rabbit Secondary Antibody (25 μl, 1mg/ml)
• 500 ml Odyssey Blocking Buffer
• 10 Odyssey Nitrocellulose Membranes (0.22 μm, 7 x 8.5 cm)

IRDye Western Kit IV (part #926-31060)
• IRDye 800CW Goat Anti-Rabbit Secondary Antibody (25 μl, 1mg/ml)
• IRDye 680 Goat Anti-Mouse Secondary Antibody (25 μl, 1mg/ml)
• 500 ml Odyssey Blocking Buffer

• 10 Odyssey Nitrocellulose Membranes (0.22 μm, 7 x 8.5 cm)

Ordering Information:

Part #	Description	Unit/Size	Downloads	Order Online
926-31050	Odyssey Western Blotting Kit I Optimized for 10 Westerns	1 Kit	msds pack insert	$ 297.00
926-31052	Odyssey Western Blotting Kit II Optimized for 10 Westerns	1 Kit	msds pack insert	$ 297.00
926-31058	Odyssey Western Blotting Kit III Optimized for 10 Westerns	1 Kit	msds pack insert	$ 297.00
926-31060	Odyssey Western Blotting Kit IV Optimized for 10 Westerns	1 Kit	msds pack insert	$ 297.00

Shipping information: U.S.A.: Same-day shipping (Federal Express overnight) available on orders received before 12:00 noon CST. Canada: Allow 3-5 days for shipments. Germany & UK: Contact LI-COR GmbH. International: Contact a LI-COR distributor in your area. All shipments subject to availability.