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LI-COR Webinar Series

LI-COR is pleased to announce their Webinar Training and Education Series.

Brought to you via:

The In Cell Western Assay: Fast, accurate measurement of protein levels with quantitative immunocytochemistry
and near-infrared fluorescence.

This webinar was broadcase live via webex on November 20, 2008.
A replay of the webinar can be seen by following this link.

Fast, accurate measurement of protein levels with quantitative immunocytochemistry and near-infrared fluorescence.

Are you studying signal transduction? Would you like to skip the Western blots and get a faster, more direct readout of what’s happening in your cultured cells?

First described in 2002, the In Cell Western assay was developed by LI-COR scientists looking for a better way to quantify proteins in cultured cells. Analyzing many samples or time points by Western blot is labor-intensive and time-consuming, and introduces the potential for error. In contrast, In Cell Western cell-based assays allow you to grow your cells in 96- or 384-well plates, treat them with the appropriate conditions, and then quickly fix them to capture a snapshot of protein expression and phosphorylation levels at that moment in time. Cells are then stained with primary and secondary antibodies against proteins of interest, in a process that is conceptually similar to immunofluorescent staining for microscopy. After staining, the total fluorescent signals from each well are imaged and quantified, allowing you to make ensemble measurements of the cell populations in each well. The ability to stain and image simultaneously in two fluorescence channels allows you to improve accuracy by normalizing for well-to-well variation in cell number – or, if you prefer, stain with antibodies against two different targets to maximize the data output from each plate. The assay format is particularly well suited to phosphoprotein and signal transduction analysis.

In this webinar, we will:

Discuss the technique, and how the images are captured and quantified
Review several options for cell number normalization
Compare and contrast this assay with other related assay formats, such as FACE and CASE
Discuss a variety of examples from the literature showing how In Cell Western assays are used
Describe the “On Cell Western” variation – ligand binding and receptor recycling assays
Demonstrate how cell-based assays can be used to validate and optimize optical imaging agents that are intended for in vivo imaging use
Review optimization tips for making the transition from traditional Western blotting or immunofluorescence experiments to In Cell Westerns
Conclude with a Q&A session to address your questions about this technology and how it could help you streamline your research

Improving Sensitivity with Odyssey Blots vs. Chemiluminescence Blots Improve your Odyssey blot performance with the tips provided in this presentation. Troubleshooting tips include: Changing software settings such as the Background, Linear Manual/Linear Auto, and Brightness and Contrast. Altering the assay chemistry by changing dilution factors, using different blocking buffers, and more.
LATEST WEBINAR: Quantitative, Multiplexed Western Blot Detection with Infrared Fluorescence – Dr. Amy Geschwender May 1, 2008 (56 minutes) This presentation discusses the differences between fluorescent and chemiluminescent Western detection, and illustrates how this technology is being used around the world. Topics include: How near-infrared fluorescence differs from other Western blotting methods Linear ranges of detection for fluorescence and chemiluminescence Multiplexed Westerns Transitioning from ECL to fluorescence How this technology is being used Quantification and normalization Phosphoprotein analysis Validation of RNAi knockdown High throughput Western blotting In-gel Western detection without transfer Reagents, instruments, and software for fluorescent Western blotting

Odyssey Infrared Imaging System - Dr. Amy Geschwender - Jun 6, 2008 Previously featured on BioCompare, Dr. Geschwender, Director of Molecular Biology @ LI-COR Biosciences, introduces the Odyssey Infrared Imaging System with particular focus on it's application in Western Blotting and it's advantages when compare with the best chemiluminescence has to offer.

Characterizing GPCR Function - Dr. Christopher Kearn - Jun 6, 2008 Dr. Christopher Kearn, University of Washington, discusses the effects of CB1 and CB2 cannabinoid receptor stimulation or inhibition and how the signaling of CB1 receptors through these various effectors is regulated.

Antibody FAQs - Dr. Kristi Ambroz - Jun 6, 2008 Watch a 9 minute question and answer presentation by Dr. Kristi Ambroz, LI-COR Senior Scientist. Questions Include: • What are the advantages of infrared detection? • What kinds of dye labeled antibodies can I use on the Odyssey/Aerius? • Where do I get infrared labeled antibodies?

Optimizing Signal Detection for Odyssey Western Blots - Jun 6, 2008 A short instruction on how to troubleshoot missing and weak Odyssey Western Blot bands.

Why Image in the NIR?
Baylor College of Medicine Seminar Series - January 30, 2008

Instrumentation and Imaging Considerations - Dr. Eva M. Sevick-Muraca

Seminar on the value of NIR imaging and how it compares to other imaging technologies.

Systematic Evaluation and Use of Targeted IRDye labeled Optical Contrast Agents - Dr. D. Michael Olive

Seminar on the systematic development of optical agents for in vivo cancer imaging.

In Vivo Imaging of Cytotoxic T Lymphocyte Homing using LI-COR IRDye 800CW Near Infrared Dye - Dr. Aaron Foster

Seminar on the use of IRDye 800CW labeled t-cells for in vivo tracking.

Nodal Staging of HER2 Positive Breast Cancer with Dual-Labeled Trastuzuman Based Imaging Agent - Lakshmi Sampath

Seminar on the use of IRDye 800CW labeled Trastuzuman for targeting Breast cancer.

NIR Imaging With the LI-COR Pearl Imager
Univ. of Nebraska Med. Center Seminar Series - April 30th, 2008

In Vivo Tracking of the BChE/IRDye 800CW Complex in BChE Knockout Mice - Ellen Duysen

Seminar highlighting the use of the LI-COR Pearl Imager for detection of IRDye labeled BChE.

Systematic Evalutaion of Targeted IRDye Labeled Optical Imaging Agents - Joy Kovar M.S.

A thorough discussion of optical agent development using the LI-COR Pearl Imager.

Illuminating Prostate Cancer Progession- Dr. Melanie Simpson

Utilization of the LI-COR Pearl Imager to measure the progression of prostate cancer in mice.

Education Series


Advances in In Vivo Imaging: Near-Infrared Optical Imaging of Mice - Jeff Harford, LI-COR Biosciences

	Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer - Dr. Eva Sevick-Muraca and Dr. Shi Ke, Baylor College of Medicine - Jun 6, 2008

	Prostate Cancer Imaging - Dr. Melanie Simpson - Jun 6, 2008 Dr. Melanie Simpson, University of Nebraska-Lincoln, discusses the challenges of in vivo research and the use of near-infrared probes for small animal imaging.

	Near Infrared Fluorescent Approaches to Cell-Based Assays and Small Animal Imaging - Dr. Amy Schutz-Geschwender - May 1, 2007 Presented at Experimental Biology, May 1, 2007

"Inquiry-based Learning in Undergraduate Genomics Education" - Dr. Cheryl Kerfeld -

Dr. Cheryl Kerfeld discusses the value of inquiry based learning (IBL).

UCLA's "Undergraduate Genomics Research Initiative" - Dr. Cheryl Kerfeld -

Dr. Cheryl Kerfeld discusses how IBL has been implemented into the undergraduate program at UCLA.

Genomics Education Matching Funds Informative Webinar - Jackie Potts

Webinar topics include:

What the GEMF program is, and how it has been modified to
better meet your needs
How research in the classroom enhances student and faculty interaction
How this award can enhance student and faculty recruitment
How this award can help your university become recognized as a leader in
undergraduate science education
Testimonials from past GEMF winners on how they have benefited from the program.

Odyssey Power Users Guide

Part 1a – Scanning

This presentation will cover many aspects associated with the set-up, naming, and previewing of scans on the Odyssey Infrared Imager. Details...

Part 1b – Scanning

This presentation will highlight how to set and edit scan and scanner parameters. Details...

Part 1c – Scanning

This presentation will cover everything from placing a sample on the Odyssey to starting and completing a scan. Details...

Part IIa – Image Adjustment

This presentation will explain how to adjust the scan image display. Details...

Part IIb – Image Adjustment

This presentation will explain several options for image orientation and placement. Details...

Part IIc – Image Adjustment

This presentation will explain several image display options and image annotation settings. Details...

Part IIIa – Analysis

This presentation will discuss quantification and concentration calculations. Details...

Part IIIb – Analysis

In this presentation we will discuss how to draw features on a scanned image. Details...

Part IIIc – Analysis

In this presentation we will discuss how to use Plug-In reports successfully. Details...

Part IVa – Reports

In this presentation we will demonstrate how to print and export reports. Details...

Part IVb – Reports

In this presentation we will discuss how to manage report templates. Details...

Part V – Image Export

In this presentation we will discuss how to export images for analysis in other programs or submission for publication. Details...

Other

Optimizing Signal Detection for Odyssey Western Blots - Jun 6, 2008

A short instruction on how to troubleshoot missing and weak Odyssey Western Blot bands.

Odyssey Infrared Imaging System - Dr. Amy Geschwender - Jun 6, 2008

Previously featured on BioCompare, Dr. Geschwender, Director of Molecular Biology @ LI-COR Biosciences, introduces the Odyssey Infrared Imaging System with particular focus on it's application in Western Blotting and it's advantages when compare with the best chemiluminescence has to offer.

Upcoming Webinars

	Glycoprotein Analysis on the Odyssey Date: TBA
	IRDye QC-1 Quencher Date: TBA Learn more about the QC-1

The In Cell Western Assay: Fast, accurate measurement of protein levels with quantitative immunocytochemistry and near-infrared fluorescence.

The In Cell Western Assay: Fast, accurate measurement of protein levels with quantitative immunocytochemistry
and near-infrared fluorescence.